Vol 2, Issue 2 (2018)

Published on 2018-04-08

In vitro antifungal activity of Bacillus subtilis against Neofusicoccum mangiferae and optimization of its growth conditions

Abstract:

Bacillus subtilis was isolated from date’s juice in Tunisia. The in vitro antifungal activity of Bacillus subtilis against Neofusicoccum mangiferae, causal agent of the disease of the decline of apricot in the oasis of southern Tunisia, was tested. An optimization of the growth conditions of Bacillus subtilus on medium based on date syrup was determined (characterizing of the temperature, pH and the composition of the nutrient medium). The results showed that the Bacillus subtilis strains inhibited 100% the mycelial growth of Neofusicoccum mangiferae, in vitro experiments on Potato Dextrose Agar medium.
The growing conditions of B. subtilis in Erlenmeyer on the basis of the maximum specific growth rate allowed us to obtain the following optimal values: pH = 7, temperature of 30 ° C, [Sugar]=10g/l, [Yeast extract] = 2.5 g/L, [(NH4)2SO4] = 5 g/L, [KH2PO4] = 2 g/L, [K2HPO4] = 1 g/L, [MgSO4,7H2O] = 0.5 g/L, and [MnSO4,7H2O] = 0.01 g/L.
So according this result, the bacteria Bacillus subtilis can be used as a biocontrol against Neofusicoccum mangiferae. Seen the strong antifungal activity of Bacillus subtilis against Neofusicoccum mangiferae, and the success of optimization Bacillus subtilis culture on medium based on date syrup, this strain can thus be used as an alternative method for integrated of decline apricot disease management in oasis.

Authors:

  • Ahmed Namsi
  • Amel Moula
  • Monia Jemni
  • Sihem Ben Maachia
  • Sofien Chniti

Keywords:

  • Neofusicoccum mangiferae
  • Antifungal activity
  • Bacillus subtilis
  • Optimization medium

Pages: -

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